海星生物
TCH-C104-UD
人膀胱癌细胞(5637)(经膀胱癌基因功能研究验证)

人膀胱癌细胞(5637)(经膀胱癌基因功能研究验证)

5637是从一位68岁白人男性II级癌患者的膀胱中分离出来的具有上皮形态的细胞系。其生物学行为(如增殖、侵袭、化疗应答)接近临床侵袭性膀胱癌表型,可模拟膀胱癌进展中的核心病理过程(如肿瘤微环境交互、耐药发生)。

产品简介

类型: 细胞株 品牌: HyCyte®
细胞别名: 5637 产品货号: TCH-C104-UD
种属: 人 规格: 1X10^6
用途: 仅供科研使用

产品参数

细胞名称:人膀胱癌细胞(经膀胱癌基因功能研究验证)

细胞简称:5637

产品货号:TCH-C104-UD

种属来源:人

组织来源:膀胱

疾病特征:膀胱癌

细胞形态:上皮细胞样

生长特性:贴壁生长

培养体系:RPMI-1640+10% FBS+1% P/S

配套培养基货号:TCH-G104

传代比例:1:3-1:4,每2-3天换液一次

倍增时间:24-48 h

培养条件:气相:95%空气+5%CO2,温度:37℃

冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存

质量检测:细菌、真菌、支原体检测均为阴性

参考文献:

Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 24381: Fogh J . Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 Bellet D , et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 32266: Bender CM , et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064 Hu SX , et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991 Schnier JB , et al. G1 arrest and down-regulation of cyclin E/cyclin-dependent kinase 2 by the protein kinase inhibitor staurosporine are dependent on the retinoblastoma protein in the bladder carcinoma cell line 5637. Proc. Natl. Acad. Sci. USA 93: 5941-5946, 1996. PubMed: 8650198

注意事项:

5637细胞较难消化,注意延长消化时间,消化到细胞收缩变圆,轻拍培养瓶侧边,细胞可以滑落方可终止消化,细胞消化时间约需要10min左右(0.25%胰酶)。

5637 已发表过的文章:

IF=5.8 Qi D, Lu Y, Qu H, et al. Independent prognostic value of CLDN6 in bladder cancer based on M2 macrophages related signature[J]. Iscience, 2024.