海星生物
TCH-C104L
荧光素酶标记的人膀胱癌细胞(5637-Luc)

荧光素酶标记的人膀胱癌细胞(5637-Luc)

该细胞系是1974年从68岁的男性膀胱癌组织中分离;在发表的文章中显示能表达几种生长因子(e.g. SCF, IL-1,IL-6,G-CSF,GM-SCF, etc.)。,所供 Luciferase 稳转细胞株采用慢病毒法构建,除稳定高效地表达 luciferase 基因外,还具有特异性强、成像质量高、发光强度可精确定量等优点,可实现包括启动子活性研究、哺乳动物细胞双杂交实验以及活体动物成像实验等多方面的灵活应用。

产品简介

类型: 荧光素酶标记细胞 品牌: HyCyte®
细胞别名: 5637 产品货号: TCH-C104L
种属: 人 规格: 1X10^6
用途: 仅供科研使用

产品参数

细胞名称:荧光素酶标记的人膀胱癌细胞

细胞简称:5637-Luc

产品货号:TCH-C104L

转染方法:慢病毒

抗性:Puro

靶细胞名称:人膀胱癌细胞

靶细胞别名:5637

种属来源:人

组织来源:膀胱

疾病特征:膀胱癌

细胞形态:上皮细胞样

生长特性:贴壁生长

培养体系:RPMI-1640+10% FBS(胎牛血清)+1.0 ug/mL Puromycin+1% P/S

配套培养基货号:TCH-G104L

传代比例:1:3-1:4,每2-3天换液一次

倍增时间:24-48 h

培养条件:气相:95%空气+5%CO2,温度:37℃

冻存条件:60%基础培养基+30%FBS+10%DMSO,液氮储存

质量检测:细菌、真菌、支原体检测均为阴性

参考文献:

Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 24381: Fogh J . Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 Bellet D , et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484 32266: Bender CM , et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064 Hu SX , et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991 Schnier JB , et al. G1 arrest and down-regulation of cyclin E/cyclin-dependent kinase 2 by the protein kinase inhibitor staurosporine are dependent on the retinoblastoma protein in the bladder carcinoma cell line 5637. Proc. Natl. Acad. Sci. USA 93: 5941-5946, 1996. PubMed: 8650198

注意事项:

5637细胞较难消化,注意延长消化时间,消化到细胞收缩变圆,轻拍培养瓶侧边,细胞可以脱落方可终止消化。

5637 已发表过的文章:

IF=5.8 Qi D, Lu Y, Qu H, et al. Independent prognostic value of CLDN6 in bladder cancer based on M2 macrophages related signature[J]. Iscience, 2024.