产品展示

细胞名称：人胚肾细胞【STR鉴定报告可咨询获取】

细胞简称：293T

产品货号：TCH-C101

种属来源：人

组织来源：胚肾

疾病特征：/

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系：DMEM-H+10%FBS+1%P/S

配套培养基货号：TCH-G101

传代比例：1:4-1:8，每2-3天换液一次

倍增时间：24-48 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

参考文献：

Letko M, et al. Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses. Nat Microbiol 5(4): 562-569, 2020. PubMed: 32094589

Sena-Esteves M, et al. Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J. Virol. 73: 10426-10439, 1999. PubMed: 10559361

Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999

Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 6,329,199 dated Dec 11 2001  

Pear WS, et al. Production of High-Titer Helper-Free Retroviruses by Transient Transfection. Proc. Natl. Acad. Sci. USA 90: 8392-8396, 1993. PubMed: 7690960

293T 已发表过的文章：

IF=14.1 Zhou LH, Gu LF, Chen YX, Jing P, Yang TT, He Y, Bao YL, Zhang XZ, Du C, Wang SB, Shan TK, Chen JY, Wang H, Wang QM, Xie LP, Zhao Y, Ji Y, Wang LS. HMGB2 Promotes Cardiomyocyte Proliferation and Heart Regeneration Through MTA2-Driven Metabolic Reprogramming. Adv Sci (Weinh). 2025 Oct 15:e05820.

IF=12.6 Tian Y, Sun D, Liu N, et al. Biomimetic mesenchymal stem cell membrane-coated nanoparticle delivery of MKP5 inhibits hepatic fibrosis through the IRE/XBP1 pathway[J]. Journal of Nanobiotechnology, 2024, 22(1): 741.

IF=10.3 Tong Y, Chen Z, Wu J, et al. METTL3 promotes an immunosuppressive microenvironment in bladder cancer via m6A-dependent CXCL5/CCL5 regulation[J]. Journal for Immunotherapy of Cancer, 2025, 13(4): e011108.

IF=9 Zhang H, Zhao Y, Wang J, et al. FBXO7, a tumor suppressor in endometrial carcinoma, suppresses INF2-associated mitochondrial division[J]. Cell Death & Disease, 2023, 14(6): 368.

IF=8 Yu Z, Wu X, Zhu J, et al. BCLAF1 binds SPOP to stabilize PD-L1 and promotes the development and immune escape of hepatocellular carcinoma[J]. Cellular and Molecular Life Sciences, 2024, 81(1): 82.

IF=6 Li P, Xi Y, Zhang Y, et al. GLA Mutations Suppress Autophagy and Stimulate Lysosome Generation in Fabry Disease[J]. Cells, 2024, 13(5): 437.

IF=3.1 Zhu H, You Y, Duan G, Yu Y, Zhang Z, Xi D, Li J, Fang M. β-galactosidase Dynamic Monitoring in Cellular Senescence Process with a Novel Activatable Fluorescence Probe. J Fluoresc. 2026 Apr 10.