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MACS® Freezing Solution 组织冻存液
The MACS Freezing Solution is a ready-to use serum-free and animal component-free media formulation developed for the cryopreservation of primary cells and solid tissues. The MACS Freezing Solution ensures high preservation of viability and recovery of cells and tissues after thawing.
产品简介
Cryopreservation of living cells and tissues is frequently applied for long term storage to preserve the samples for later use. The freezing and thawing of the samples are critical steps in the cryopreservation process which can influence the recovery of viable cells after storage. The MACS Freezing Solution allows the cryopreservation of primary cells and solid tissue samples under serum-free conditions with high preservation of viability and recovery of cells and tissues after thawing. It has been tested and validated on diverse cell types and tissues, such as human and mouse tumor samples, peripheral blood mononuclear cells (PBMCs), and cells from dissociated solid tissues (e.g. cells from dissociated tumors). MACS Freezing Solution contains 10% DMSO as cryoprotectant.
产品参数
Analysis of cryopreserved PBMCs. Peripheral blood mononuclear cells (PBMCs) from 3 different donors were cryopreserved for 2 weeks in liquid nitrogen, either using RPMI containing 20% FCS and 10% DMSO, or the MACS Freezing Solution. After this time period, cells were thaw and cell recovery (A) and cell viability (B) were analyzed by flow cytometry using propidium iodide staining and a MACSQuant Analyzer 10. Cell recovery is calculated as the percentage of viable cells recovered after thawing with reference to the amount of fresh cells that were cryopreserved.
