美天旎
130‑096‑535
产品简介
The Pan T Cell Isolation Kit has been developed for the faster isolation of untouched T cells from human peripheral blood mononuclear cells (PBMCs). This updated kit gives you even higher purity, greater recovery, and faster cell isolation. You can isolate untouched T cells in as little as 18 minutes!
Detailed separation procedure
Non-target cells, i.e., monocytes, neutrophils, eosinophils, B cells, stem cells, dendritic cells, NK cells, granulocytes, or erythroid cells are labeled by using a cocktail of biotin-conjugated antibodies. The cocktail contains antibodies against CD14, CD15, CD16, CD19, CD34, CD36, CD56, CD123, and CD235a (Glycophorin A). Subsequently, non-target cells are magnetically labelled with the Pan T Cell MicroBead Cocktail. Isolation of highly pure T cells is achieved by depletion of magnetically labelled cells.
Downstream applications
T cells isolated with the Pan T Cell Isolation Kit can be used when direct labeling of T cell surface molecules could interfere with downstream applications, e.g., in studies on T cell activation.1, 2 T cells isolated with the Pan T Cell Isolation Kit have also been used to generate antigen-specific T cell lines.3, 4 Untouched T cells may also be used for further isolation of specific T cell subsets, such as naive5 or memory T cells and activated T cells.
Columns
LS or autoMACS® Columns.
产品参数
Figure 1
Separation of untouched T cells from PBMCs by depletion of non-T cells using the Pan T Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were labeled with CD2-PE and CD3-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer.
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PBMCs before separation
Figure 2
Isolation of untouched T cells.
