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This assay employs the quantitative sandwich enzyme immunoassay technique. Anantibody specific for IL-10 has been pre-coated onto a microplate. Standards andsamples are pipetted into the wells and any IL-10 present is bound by the immobilizedantibody. After washing away any unbound substances, an enzyme-linked antibodyspecific for IL-10 is added to the wells. Following a wash to remove any unboundantibody-enzyme reagent, TMB substrate solution (Chromogenic agent) is addedtothe wells and color develops in proportion to the amount of IL-10 bound in the initial
step. The color development is stopped and the intensity of the color is measured.