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This assay employs the quantitative sandwich enzyme immunoassay technique. Amonoclonal antibody specific for human IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any human IFN-γ present isbound by the immobilized antibody. After washing away any unbound substances, anenzyme-linked monoclonal antibody specific for human IFN-γ is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, TMBsubstratesolution (Chromogenic agent) is added to the wells and color develops in proportiontothe amount of human IFN-γ bound in the initial step. The color development is stopped, and the intensity of the color is measured.