abcam
ab119216
Bradford蛋白定量
Bradford Reagent (ab119216) is a quick, ready-to-use colorimetric method for total protein quantification. - Improved protein dye formulation for protein quantification, offering higher tolerance to detergent (up to 1%) - Reduced protein-to-protein variation compared to traditional Bradford assays - Used to make standard curves to quantify both low protein range (1 µg/mL – 25 µg/mL) or high protein range (0.1 mg/mL – 1.5 mg/mL) - Proven performance: cited in over 40 publications
产品简介
This product is manufactured by Expedeon, an Abcam company, and was previously called Bradford Ultra - 500 ml. BFU05L is the same as the 500 ml size.
Bradford Reagent ab119216 offers an improved formulation over classical Bradford: it has higher tolerance of detergent contamination of the protein samples.
This Bradford Reagent shows excellent linearity for a defined range of protein concentrations and shows significantly less protein-to-protein variation than is observed with other Bradford-type Coomassie formulations.
When Coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue. Protein concentrations are estimated by reference to the absorbance obtained for a series of standard protein dilutions, which are assayed alongside samples with unknown protein concentrations.
Manufactured under patent numbers: US 12/226,797 ; SG 201103010-3 ; SG 200807953-5 ; JP 2009-507182 ; IN 5722/CHENP/2008 ; GB 0608377.8 ; EP 10187154.9; EP 07733647.7 ; CN 200780021434.8 ; CA 2,649,835 ; AU 2007245443.
Support on how to determine protein concentration can be found here.
产品参数
性能和储存信息
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
